Open AccessScreening a novel FGF3 antagonist peptide with anti-tumor effects on breast cancer from a phage display library
Author(s) -
Wei Wang,
Tao Chen,
Haicheng Li,
Yuhui Chen,
ZhiYong Wu,
Tongming Feng,
Xilin Zhang,
Qian Zhong,
Qianhong Zhong,
Guozhou Li,
Liang Guo,
Lin Zhou,
Jie Zhou
Publication year - 2015
Publication title -
molecular medicine reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.727
H-Index - 56
eISSN - 1791-3004
pISSN - 1791-2997
DOI - 10.3892/mmr.2015.4248
Subject(s) - biology , cell cycle , oncogene , phage display , cell growth , cancer research , protein kinase b , cancer , peptide , cell , microbiology and biotechnology , signal transduction , biochemistry , genetics
Accumulating evidence has suggested that fibroblast growth factor 3 (FGF3) is expressed in breast cancer and correlates with the stage and grade of the disease. In the present study, a specific FGF3‑binding peptide (VLWLKNR, termed FP16) was isolated from a phage display heptapeptide library with FGF3. The peptide FP16 contained four identical (WLKN) amino acids and demonstrated high homology to the peptides of the 188‑194 (TMRWLKN) site of the high‑affinity FGF3 receptor fibroblast growth factor receptor 2. Functional analyses indicated that FP16 mediated significant inhibition of FGF3‑induced cell proliferation, arrested the cell cycle at the G0/G1 phase by increasing proliferation‑associated protein 2G4, suppressing cyclin D1 and proliferating cell nuclear antigen, and inhibited the FGF3‑induced activation of extracellular signal‑regulated kinase 1/2 and Akt kinase. Taken together, these results demonstrated that the peptide FP16, acting as an FGF3 antagonist, is a promising therapeutic agent for the treatment of breast cancer.