Open AccessADAM17 silencing suppresses the migration and invasion of non-small cell lung cancer
Author(s) -
Xiaoyi Lv,
Li Yang,
Ming Qian,
Chao Ma,
Hongyu Jing,
Wen Zhang,
Donghua Qian
Publication year - 2014
Publication title -
molecular medicine reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.727
H-Index - 56
eISSN - 1791-3004
pISSN - 1791-2997
DOI - 10.3892/mmr.2014.2029
Subject(s) - cell cycle , downregulation and upregulation , small hairpin rna , cell growth , cancer research , gene silencing , biology , cell , oncogene , a549 cell , small interfering rna , cell culture , a431 cells , cell migration , rna interference , tumor progression , cancer , microbiology and biotechnology , transfection , rna , gene knockdown , biochemistry , genetics , gene
A disintegrin and metalloprotease (ADAM) 17 has been implicated in the tumor progression of various types of solid tumor; however, little is known about its role in non-small cell lung carcinoma (NSCLC). The present study evaluated whether the downregulation of ADAM17 affects cell proliferation, the cell cycle, cell migration and cell invasion in NSCLC. A recombinant lentiviral small hairpin RNA (shRNA) expression vector carrying ADAM17 was constructed and then infected into A549 cells, a human NSCLC cell line. Cell proliferation, cell cycle progression, cell migration and cell invasion were determined following the downregulation of ADAM17 by siRNA. It was revealed that downregulation of ADAM17 expression using an RNA silencing approach in A549 tumor cells significantly suppressed cell proliferation and invasion in vitro, and tumor growth in vivo. These data suggested that ADAM17 is an important regulator of the tumorigenic properties of human NSCLC and may be used as a potential anticancer therapeutic target in NSCLC.