Open AccessEffects of cyclin E gene silencing on the proliferation of esophageal cancer cell lines, EC9706, Eca109 and KYSE30
Author(s) -
Na Wang,
Min Li,
Wenqiao Zang,
Yunyun Ma,
Yuanyuan Wang,
Guoqiang Zhao
Publication year - 2013
Publication title -
molecular medicine reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.727
H-Index - 56
eISSN - 1791-3004
pISSN - 1791-2997
DOI - 10.3892/mmr.2013.1280
Subject(s) - cell cycle , transfection , microbiology and biotechnology , biology , gene silencing , cell growth , cyclin e , cyclin b1 , cyclin b , cyclin d1 , oncogene , cyclin , cyclin a , flow cytometry , cyclin d , apoptosis , cancer research , cell culture , gene , cyclin dependent kinase 1 , genetics
In order to observe the effects of cyclin E gene silencing by small interferingRNA (siRNA) on the growth, proliferation, invasion and apoptosis of esophagealcancer cell lines, including EC9706, Eca109 and KYSE30, siRNA vectors targetingcyclin E gene were constructed and then transfected into the EC9706, Eca109 andKYSE30 human esophageal cancer cell lines. Cyclin E mRNA and protein expressionwere determined by RT-PCR and western blotting. Cell proliferation and clonalitywere detected using a CCK-8 test and soft agar colony formation assay. Cell cycledistribution, apoptosis and invasion of EC9706, Eca109 and KYSE30 cells were evaluatedwith flow cytometry and a transwell culture system. After siRNA vectors targetingthe cyclin E gene were transfected into EC9706, Eca109 and KYSE30 cell lines,compared with blank and negative control groups, the expression of cyclin E mRNAand protein (P<0.01), colony-forming units and the number of cells penetratingthe transwell membrane (P<0.05) were significantly decreased, the cells inthe S and G2/M phase were reduced, the cells in the G0/G1 phase were increasedand the apoptosis rate was increased (P<0.01) in the experimental groups. Cyclin Egene silencing effectively inhibits growth, proliferation and invasion of esophagealcancer cells.