Open AccessIdentification of GIRK2-4 subunits in human esophageal smooth muscle cells
Author(s) -
Qiang Lü,
Li Gong,
Hui Xu,
Tao Zhang,
Xiaolong Yan,
Jinbo Zhao,
Zhipei Zhang,
Yunjie Wang,
Han Ye
Publication year - 2011
Publication title -
molecular medicine reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.727
H-Index - 56
eISSN - 1791-3004
pISSN - 1791-2997
DOI - 10.3892/mmr.2011.499
Subject(s) - molecular medicine , vagus nerve , esophagus , messenger rna , oncogene , biology , interstitial cell of cajal , protein subunit , acetylcholine , smooth muscle , blot , microbiology and biotechnology , cell , endocrinology , anatomy , cell cycle , biochemistry , gene , stimulation
Acetylcholine (ACh) secreted from the vagus nerve contributes to the physiological and pathological regulation of the contraction and relaxation of human esophageal smooth muscle. Expression of acetylcholine-sensitive G protein‑activated inwardly rectifying potassium channels (GIRKs) occurs widely in the heart, nervous system and gastrointestine, but the role of GIRKs in the esophagus remains unclear. In the present study, expression of the GIRK1-4 subunits in mRNA and total protein was examined in human esophageal smooth muscle cells (SMCs) by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. mRNA and protein expression of the GIRK2-4 subunits was detected in human esophageal longitudinal muscle (LM) and circular muscle (CM) cells. However, GIRK1 mRNA and protein were not observed in either the esophageal LM or CM. This study is the first to identify the expression of GIRK2-4 subunits in human esophageal SMCs.