Open AccessKnockdown of microRNA‑29a regulates the expression of apoptosis‑related genes in MCF‑7 breast carcinoma cells
Author(s) -
Gholamreza Khamisipour,
Elham Mansourabadi,
Behrouz Naeimi,
Ali Moazzeni,
Rahim Tahmasebi,
Mojtaba Hasanpour,
Majid Mosahebi Mohammadi,
Zahra Mansourabadi,
Shakib Shamsian
Publication year - 2017
Publication title -
molecular and clinical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.442
H-Index - 7
eISSN - 2049-9469
pISSN - 2049-9450
DOI - 10.3892/mco.2017.1528
Subject(s) - survivin , gene knockdown , microrna , biology , apoptosis , cancer research , mcf 7 , oncogene , transfection , cell cycle , downregulation and upregulation , cell culture , cancer cell , cancer , gene , genetics , human breast
MicroRNA (miR), as non-coding small RNA, are key regulators of cancer-related biological cell processes and contribute to tumor growth through regulation of groups of pro- and anti-apoptotic genes. The present study aimed to investigate the effects of miR-29a on the expression of genes involved in apoptosis, including p21, B-cell lymphoma 2 (BCL-2), p53 and survivin. The MCF-7 breast cancer cell line was transfected with anti-miR-29a and treated with Taxol in subdivided treatment groups including: Scramble; anti-miR-29a; anti-miR-29a + Taxol; Taxol; and control. Expression levels of p21, BCL-2, p53 and survivin were evaluated using reverse transcription-quantitative polymerase chain reaction. miR-29a knockdown resulted in p21 and p53 upregulation and a decrease in survivin expression. These results indicated that miR-29a inhibition regulates apoptosis. The present data suggested that miR-29a inhibition may be a promising strategy for the induction of apoptosis of tumor cells.