
Mitotic catastrophe induced in HeLa cells by photodynamic treatment with Zn(II)-phthalocyanine
Author(s) -
Santiago Rello-Varona,
Juan C. Stockert,
Magdalena Cañete,
Pilar Acedo,
Ángeles Villanueva
Publication year - 1992
Publication title -
international journal of oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.405
H-Index - 122
ISSN - 1019-6439
DOI - 10.3892/ijo_32_6_1189
Subject(s) - mitotic catastrophe , hela , photodynamic therapy , programmed cell death , apoptosis , mitosis , biology , cell cycle , cell , metaphase , cancer research , microbiology and biotechnology , necrosis , chemistry , biochemistry , genetics , organic chemistry , gene , chromosome
Photodynamic therapy (PDT) is a tool against neoplastic and non-neoplastic diseases. PDT is capable to induce different cell death mechanisms in vitro, triggered in a dose-dependent manner. Relationships between PDT and apoptosis or necrosis induction are well-known, but other cell death mechanisms triggered after PDT are less understood. Here we present our results in p53-deficient human cervix carcinoma HeLa cells subjected to sublethal PDT treatments (mortality about 40%) using Zn(II)-phthalocyanine (ZnPc) incorporated into liposomes. We obtained a rapid metaphase blockage of cells that also showed clearly altered configurations of the mitotic spindle. Cell cycle arrest was followed by aneuploidisation and cell death with apoptotic morphology. Apoptosis was also confirmed by occurrence of PARP cleavage and Bax translocation to mitochondria. These features are components of the cell death mechanism known as mitotic catastrophe and represent, to our knowledge, the first description of this cell death modality after PDT with ZnPc.