Open AccessMetastatic effect of LY-6K gene in breast cancer cells
Author(s) -
HaeSim Park
Publication year - 2009
Publication title -
international journal of oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.405
H-Index - 122
ISSN - 1019-6439
DOI - 10.3892/ijo_00000371
Subject(s) - urokinase receptor , cancer research , metastasis , biology , breast cancer , cancer , oncogene , cancer cell , cell cycle , cell migration , cell , genetics
The over-expression of urokinase receptor in breast cancer cells results in increased tumor invasion, growth and metastasis. As LY-6K belongs to the Ly6/uPAR superfamily, we assessed the association of LY-6K with metastasis in breast cancer cell lines and how the LY-6K gene mediates cancer cell metastasis. The migration ability of cells in which expression of LY-6K gene is up- or down-regulated was investigated by transfecting LY-6K naked DNA and treating LY-6K siRNA. Assays of wound healing, migration and invasion were performed. The assays demonstrated that cell migration significantly increased when LY-6K gene is over-expressed or LY-6K protein is abnormally expressed in breast cancer cells. In addition, LY-6K caused cancer cell metastasis and it is linked to the Ras/ERK signaling pathway. Our results suggest that LY-6K meaningfully participates in breast cancer cell metastasis by influencing cell migration and invasion through the Ras/ERK pathway.