Open AccessHBV triggers APOBEC2 expression through miR‑122 regulation and affects the proliferation of liver cancer cells
Author(s) -
Aimei Li,
Jing Wu,
Aixia Zhai,
Jun Qian,
Xinyang Wang,
Majjid A. Qaria,
Qingmeng Zhang,
Yujun Li,
Yong Fang,
Wenping Kao,
Wuqi Song,
Zhiyi Zhang,
Fengmin Zhang
Publication year - 2019
Publication title -
international journal of oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.405
H-Index - 122
ISSN - 1019-6439
DOI - 10.3892/ijo.2019.4870
Subject(s) - oncogene , biology , microrna , carcinogenesis , liver cancer , hepatitis b virus , cancer research , cancer , hbx , cell cycle , molecular medicine , mir 122 , three prime untranslated region , messenger rna , untranslated region , microbiology and biotechnology , virology , hepatocellular carcinoma , gene , virus , genetics
Hepatitis B virus (HBV) infection is responsible for 50% of liver cancer cases globally; this disease is one of the leading causes of cancer‑associated mortality. One reported mechanism underlying the development of liver cancer is the mutation of tumor suppressor genes induced by the overexpression of apolipoprotein B mRNA‑editing enzyme catalytic subunit 2 (APOBEC2) in hepatocytes. In addition, it has been observed that HBV inhibited microRNA (miR)‑122 expression in hepatocytes; however, the molecular mechanisms involved in liver cancer development remain unknown and further investigations are required. In the present study, the mechanistic roles of HBV infection in modulating the expression of miR‑122 and APOBEC2, and the development of liver cancer, were investigated. Reverse transcription‑quantitative PCR and western blot analyses revealed that APOBEC2 expression was markedly upregulated following HBV infection. Of note, the expression profile of APOBEC2 in the Huh7 and HepG2 liver cancer cell lines opposed that of miR‑122; this miR is the most abundant miRNA in the liver and has been associated with hepatocarcinogenesis. Mechanistically, it was demonstrated via a dual‑luciferase assay that miR‑122 could specifically bind to the 3'‑untranslated region (3'UTR) of APOBEC2 mRNA, inhibiting its expression. Collectively, the findings of the present study may provide insight into the mechanistic role of HBV infection in modulating the expression of miR‑122, which targets the 3'UTR of APOBEC2 mRNA, subsequently inducing liver carcinogenesis.