Open AccessRBM3 upregulates ARPC2 by binding the 3'UTR and contributes to breast cancer progression
Author(s) -
Ping Chen,
Xiaoli Yue,
Hongbing Xiong,
Xiaohong Lü,
Zhenling Ji
Publication year - 2019
Publication title -
international journal of oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.405
H-Index - 122
ISSN - 1019-6439
DOI - 10.3892/ijo.2019.4698
Subject(s) - oncogene , breast cancer , cancer research , gene knockdown , metastasis , biology , cancer , immunohistochemistry , cell growth , cell cycle , cell , molecular medicine , oncology , medicine , apoptosis , immunology , biochemistry , genetics
Breast cancer is one of the most common types of cancers which results in a high mortality rate for patients worldwide. In this study, we performed systematical experiments including tissue analysis (immunohistochemistry etc.) and cell functional experiments (cell counting assay, MTT assay, cell colony formation, cell migration assay, cell invasion assay etc.). We demonstrated that the expression level of RNA binding motif protein 3 (RBM3) was higher in human breast cancer tissues compared with adjacent non‑tumor tissues. A high level of RBM3 was associated with worse post‑operative relapse‑free survival (RFS) and overall survival (OS) rates in patients with breast cancer. Among the patients with breast cancer, the expression of RBM3 was associated with patient lymph node metastasis and a high tumor grade. The knockdown of RBM3 markedly decreased the proliferation and metastasis of human breast cancer cells. In downstream pathway analysis, actin related protein 2/3 complex subunit 2 (ARPC2) was determined to be positively regulated by RBM3 through a post‑transcriptional 3'UTR‑binding manner. ARPC2 also played an oncogenic role and mediated the promoting role of RBM3 in the proliferation and metastasis of human breast cancer cells. Thus, on the whole, the findings of this study demonstrate that RBM3 acts as an oncogene in human breast cancer cells and that the functional depletion of RBM3 may be considered as a potential method for breast cancer therapy.