Open AccessSMC1A promotes growth and migration of prostate cancer in vitro and in vivo
Author(s) -
Xiuwu Pan,
Sishun Gan,
Jian-Qing Ye,
Yong Fan,
Υi Hong,
Chuanmin Chu,
Yi Gao,
Lin Liu,
Xi Liu,
Lu Chen,
Yi Huang,
Hong Xu,
Junlong Ren,
Yin Li,
Fajun Qu,
Hai Huang,
Xingang Cui,
Danfeng Xu
Publication year - 2016
Publication title -
international journal of oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.405
H-Index - 122
ISSN - 1019-6439
DOI - 10.3892/ijo.2016.3697
Subject(s) - du145 , lncap , biology , prostate cancer , cancer research , gene knockdown , cell cycle , cell growth , oncogene , cancer , microbiology and biotechnology , cell culture , genetics
Structural maintenance of chromosome 1 alpha (SMC1A) gene has been reported to be related to tumor development in some types of human cancers. However, the misregulation of SMC1A and its functions in castration-resistant prostate cancer (CRPC) have not been well understood. In the present study, we found that SMC1A was elevated in androgen-independent PCa cell lines PC-3 and DU-145 compared to androgen sensitive LNCap and 22RV1 cells by qPCR and western blot assay. Knockdown of SMC1A inhibited cell growth, colony formation and cell migration abilities of PC-3 and DU145 cells by MTT, colony formation and transwell assays, and affected cell cycle progression in PC-3 and DU145 cells by flow cytometry. Moreover, SMC1A knockdown significantly reduced tumor growth in vivo in a nude mouse model. Additionally, we also found that the expression of SMC1A gene was higher in prostate cancer tissues than in the adjacent normal tissues by immunohistochemical staining, and was positively correlated to tumor metastasis and recurrence by Oncomine database mining. Taken together, the present study indicates that SMC1A may play an important role in malignant transformation of PCa under conditions of androgen deprivation and act as a new target for PCa diagnosis and treatment.