Open AccessPAX8 is transcribed aberrantly in cervical tumors and derived cell lines due to complex gene rearrangements
Author(s) -
Eduardo López–Urrutia,
Abraham PedrozaTorres,
Jorge FernándezRetana,
David Cantu De Leon,
Fermín Morales-González,
Nadia JacoboHerrera,
Oscar PeraltaZaragoza,
Jorge García-Méndez,
Verónica García-Castillo,
Osvaldo Bautista-Isidro,
Carlos PérezPlasencia
Publication year - 2016
Publication title -
international journal of oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.405
H-Index - 122
ISSN - 1019-6439
DOI - 10.3892/ijo.2016.3515
Subject(s) - pax8 , biology , alternative splicing , gene isoform , carcinogenesis , cancer research , cancer , renal cell carcinoma , pathology , gene , genetics , transcription factor , medicine
The transcription factor PAX8, a member of the paired box-containing gene family with an important role in embryogenesis of the kidney, thyroid gland and nervous system, has been described as a biomarker in tumors of the thyroid, parathyroid, kidney and thymus. The PAX8 gene gives rise to four isoforms, through alternative mRNA splicing, but the splicing pattern in tumors is not yet established. Cervical cancer has a positive expression of PAX8; however, there is no available data determining which PAX8 isoform or isoforms are present in cervical cancer tissues as well as in cervical carcinoma-derived cell lines. Instead of a differential pattern of splicing isoforms, we found numerous previously unreported PAX8 aberrant transcripts ranging from 378 to 542 bases and present in both cervical carcinoma-derived cell lines and tumor samples. This is the first report of PAX8 aberrant transcript production in cervical cancer. Reported PAX8 isoforms possess differential transactivation properties; therefore, besides being a helpful marker for detection of cancer, PAX8 isoforms can plausibly exert differential regulation properties during carcinogenesis.