Open AccessAKT serine/threonine protein kinase modulates baicalin-triggered autophagy in human bladder cancer T24 cells
Author(s) -
ChiTsai Lin,
Shih Chang Tsai,
Michael T. Tseng,
ShuFen Peng,
Sheng Chu Kuo,
Lin Meng,
Yu-Chin Hsu,
Miau Rong Lee,
Sakae Amagaya,
Wen Wen Huang,
Tian Shung Wu,
Jai Sing Yang
Publication year - 2013
Publication title -
international journal of oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.405
H-Index - 122
ISSN - 1019-6439
DOI - 10.3892/ijo.2013.1791
Subject(s) - baicalin , protein kinase b , scutellaria baicalensis , autophagy , pi3k/akt/mtor pathway , apoptosis , biology , cell growth , microbiology and biotechnology , cancer cell , chemistry , biochemistry , cancer , medicine , genetics , high performance liquid chromatography , alternative medicine , chromatography , traditional chinese medicine , pathology
Baicalin is one of the major compounds in the traditional Chinese medicinal herb from Scutellaria baicalensis Georgi. We investigated the molecular mechanisms of cell autophagy induced by baicalin in human bladder cancer T24 cells. Baicalin inhibited cell survival as shown by MTT assay and increased cell death by trypan blue exclusion assay in a concentration-dependent manner. Baicalin did not induce apoptotic cell death in T24 cells by TUNEL and caspase-3 activity assay. Baicalin induced the acidic vesicular organelle cell autophagy marker, manifested by acridine orange (AO) and monodansylcadaverine (MDC) staining and cleavage of microtubule-associated protein 1 light chain 3 (LC3). The protein expression levels of the Atg 5, Atg 7, Atg 12, Beclin-1 and LC3-II were upregulated in T24 cells after baicalin treatment. Inhibition of autophagy by 3-methyl-adenine (an inhibitor of class III phosphatidylinositol-3 kinase; 3-MA) reduced the cleavage of LC3 in T24 cells after baicalin treatment. Furthermore, protein expression levels of phospho-AKT (Ser473) and enzyme activity of AKT were downregulated in T24 cells after baicalin treatment. In conclusion, baicalin triggered cell autophagy through the AKT signaling pathway in T24 cells.