Open AccessSuppressing serum response factor inhibits invasion in cervical cancer cell lines via regulating Egr‑1 and epithelial-mesenchymal transition
Author(s) -
Liya Ma,
Ying Yu,
Xiao-hui Qu
Publication year - 2018
Publication title -
international journal of molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.048
H-Index - 90
eISSN - 1791-244X
pISSN - 1107-3756
DOI - 10.3892/ijmm.2018.3954
Subject(s) - epithelial–mesenchymal transition , oncogene , cell growth , serum response factor , cancer research , cell cycle , biology , cervical cancer , cell , cancer , gene knockdown , gene silencing , mesenchymal stem cell , transcription factor , cell culture , microbiology and biotechnology , metastasis , gene , biochemistry , genetics
Serum response factor (SRF) is a transcription factor that has important roles in tumor progression. However, its role in cervical cancer cell proliferation and invasion remains unclear. The present study revealed that SRF silencing constrained cervical cancer cell proliferation and invasion via controlling early growth response‑1 (Egr‑1). The results demonstrated that SRF was significantly increased in cervical cancer tissues and cell lines, compared with normal. Suppressing SRF, by using a loss‑of‑function experiment, constrained cervical cancer cell proliferation, invasion, and epithelial‑mesenchymal transition. Furthermore, SRF knockdown significantly downregulated Egr‑1 expression in cervical cancer cell lines, and overexpression of Egr‑1 reversed the effect of SRF on cell proliferation, invasion, and epithelial‑mesenchymal transition. Therefore, SRF may control cell proliferation and invasion by regulating Egr‑1 in cervical cancer.