Open AccessIdentification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2
Author(s) -
Ying Zheng,
Shudong Sheng,
Haiyan Wang,
Xin Jia,
Yanqiu Hu,
Yayun Qian,
Yongze Zhu,
Jianjun Wang
Publication year - 2012
Publication title -
international journal of molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.048
H-Index - 90
eISSN - 1791-244X
pISSN - 1107-3756
DOI - 10.3892/ijmm.2012.1065
Subject(s) - stat2 , immunoprecipitation , biology , hek 293 cells , two hybrid screening , protein subunit , protein–protein interaction , subcellular localization , microbiology and biotechnology , complementary dna , yeast , dna polymerase , dna , cell cycle , genetics , gene , stat3 , stat
Pold2 is a subunit of the DNA polymerase δ complex, encoding a protein involved in DNA replication and repair. In this study, using a yeast two-hybrid screening technique and the common cDNA fragment of the mouse PIAS2 as a bait, Pold2 was found to interact with PIAS2. A direct interaction between Pold2 and PIAS2 was confirmed by direct yeast two-hybrid. In vivo evidence of Pold2 association with PIAS2 was obtained by co-immunoprecipitation using HEK-293 cells. Subcellular localization studies demonstrated that Pold2 and PIAS2 were partially co-localized in mammalian cells. Collectively, our results suggest that Pold2 interacts under physiological conditions with PIAS2.