Open AccessEffects of lentinan on NF‑κB activity in the liver of burn rats with sepsis
Author(s) -
Qun Lv,
Yutong Gu,
Yue Qi,
Zhenjun Liu,
MA Gertz
Publication year - 2020
Publication title -
experimental and therapeutic medicine
Language(s) - English
Resource type - Journals
eISSN - 1792-1015
pISSN - 1792-0981
DOI - 10.3892/etm.2020.8955
Subject(s) - lentinan , sepsis , medicine , h&e stain , lipopolysaccharide , western blot , liver injury , tumor necrosis factor alpha , intraperitoneal injection , pharmacology , chemistry , immunohistochemistry , biochemistry , polysaccharide , gene
Effects of lentinan on nuclear factor-κB (NF-κB) activity in liver of burn rats with sepsis were investigated. To mimic the clinical sepsis after burn, rats were subjected to 30% full-thickness scald injury, followed by intraperitoneal (i.p.) injection of lipopolysaccharide (LPS). Seventy-two adult rats were randomly divided into six groups: the normal control group (n=12); the burn sepsis group (n=12); the burn sepsis with positive drugs; the burn sepsis with low-dose lentinan treatment group (50.0 mg/kg, n=12); the burn sepsis with middle-dose lentinan treatment group (100.0 mg/kg, n=12) and the burn sepsis with high-dose lentinan treatment group (200.0 mg/kg, n=12). Expression of NF-κB in the liver was measured with western blot analysis. The morphology of liver was evaluated with hematoxylin and eosin staining. The expression of NF-κB significantly increased in the liver of burn rats with sepsis. Compared with the burn sepsis group, lentinan treatment obviously reduced the damage of hepatic cell morphology, and decreased the activity of NF-κB significantly in the medium and high concentrations of lentinan treatment groups (P<0.05). Most importantly, treatment with lentinan was able to reverse the increased concentration of IL-4, IL-6, IL-10 and TNF-α in plasma which was induced by LPS. Lentinan treatment can significantly decrease the expression of NF-κB in the liver of burn rats with sepsis.