Open AccessMicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a
Author(s) -
Yiming Zhang,
Zhao Liu,
Min Zhou,
Changjian Liu
Publication year - 2016
Publication title -
experimental and therapeutic medicine
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1792-1015
pISSN - 1792-0981
DOI - 10.3892/etm.2016.3672
Subject(s) - oncogene , cell cycle , microrna , molecular medicine , cell , cell growth , vascular smooth muscle , wnt5a , microbiology and biotechnology , apoptosis , cancer research , biology , smooth muscle , signal transduction , gene , wnt signaling pathway , endocrinology , genetics
Aberrant smooth muscle cells (SMCs) play important roles in the formation of abdominal aortic aneurysm (AAA). Although the molecular mechanism of AAA formation has been investigated, there is a lack of understanding concerning the role of microRNAs (miRNAs) in AAA, which the current study aimed to address. Firstly, miRNA array analysis was performed in order to compare the miRNA profiles in a mouse model of AAA with those in normal control mice, and differentially expressed miRNAs were identified. miR-129-5p was selected for further analysis, and was used to transfect human SMCs. The results of an MTT assay revealed that miR-129-5p inhibited the proliferation of SMCs, and flow cytometry indicated that apoptosis was induced. Bioinformatic analysis predicted that Wnt5a was the potential target gene of miR-129-5p, and this was verified by luciferase assay. In summary, miR-129-5p inhibits cellular proliferation, induces apoptosis and modulates the Wnt5a signaling pathway in SMCs.