Open AccessConstruction of tissue-engineered bone using a bioreactor and platelet-rich plasma
Author(s) -
Weiliang Dong,
Honglei Jiang,
Shuzhen Wang,
Huibo Li,
Huawu Zhang,
Lei Zhao,
Tao Peng,
Zhong Cao,
Shui Sun
Publication year - 2014
Publication title -
experimental and therapeutic medicine
Language(s) - English
Resource type - Journals
eISSN - 1792-1015
pISSN - 1792-0981
DOI - 10.3892/etm.2014.1774
Subject(s) - mesenchymal stem cell , cd31 , tissue engineering , extracellular matrix , chemistry , angiogenesis , bone marrow , biomedical engineering , platelet rich plasma , bioreactor , microbiology and biotechnology , pathology , platelet , biology , in vitro , immunology , medicine , cancer research , biochemistry , organic chemistry
The aim of the present study was to construct tissue-engineered bone using a bioreactor and platelet-rich plasma (PRP). Bone marrow mesenchymal stem cells (BMSCs) and β-tricalcium phosphate (β-TCP) were cultured in a perfusion bioreactor with PRP-containing medium for 21 days to form a BMSC-TCP composite. Rabbits were then implanted with the BMSC-TCP composite. The morphology of the implanted BMSC-TCP composite was observed three months after surgery by scanning electron microscopy and hematoxylin and eosin (H&E) staining. In addition, the expression of cluster of differentiation (CD)31 and von Willebrand factor (WF) in the implanted BMSC-TCP composite was detected using immunohistochemistry. Bone formation was determined by comprehensive testing Following culture in a perfusion bioreactor and PRP, the BMSCs adhered to the β-TCP scaffold and the secretion of extracellular matrix was observed. The spreading and proliferation of cells was found to be enhanced on the scaffold. Furthermore, the vascular endothelial cell markers CD31 and VEF, were positively expressed. Therefore, these results suggest that tissue-engineered bone may be constructed using a bioreactor and PRP. PRP, which contains multiple growth factors, may promote vascularization of tissue-engineered bone.