Open AccessImprovement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion
Author(s) -
Ping Zhang,
Zhen Huang,
Haiying Yan,
Lin-Lin Su,
Yongkun Gui,
Haipeng Lv,
Bin Zhu,
Tong Li
Publication year - 2014
Publication title -
experimental and therapeutic medicine
Language(s) - English
Resource type - Journals
eISSN - 1792-1015
pISSN - 1792-0981
DOI - 10.3892/etm.2014.1483
Subject(s) - fibrous joint , pathological , common carotid artery , internal carotid artery , medicine , ischemia , pathology , carotid arteries , staining , right common carotid artery , anatomy , surgery
The aim of the present study was to provide a simple method of establishing a rat model for focal cerebral ischemia-reperfusion (FCIR). The suture-occluded method was used to establish FCIR in male Sprague-Dawley rats. An incision was made over the bifurcation of the common carotid artery (CCA), through which a suture was inserted up to the internal carotid artery (ICA). The suture remained in the skin subsequent to model establishment and was withdrawn to the CCA to enable reperfusion. The reliability of the rat model was assessed via analysis of nerve function, tetrazolium (TTC) staining and pathological examination. Following FCIR in rats, the resulting neurological impairments were observed. TTC staining revealed infarcts and pathological examination revealed typical pathological changes. This modified method was simple, reliable and, therefore, may be used to investigate FCIR.