Effects of the antihypertensive drug benidipine on osteoblast function in vitro | Zendy

Baixiang Wang | Zendy; Ming Bi | Zendy; Zhen Zhu | Zendy; Lei Wu | Zendy; Jingyun Wang | Zendy

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Effects of the antihypertensive drug benidipine on osteoblast function in vitro

Author(s) -

Baixiang Wang,

Ming Bi,

Zhen Zhu,

Lei Wu,

Jingyun Wang

Publication year - 2014

Publication title -

experimental and therapeutic medicine

Language(s) - English

Resource type - Journals

eISSN - 1792-1015

pISSN - 1792-0981

DOI - 10.3892/etm.2014.1475

Subject(s) - runx2 , osteoblast , alkaline phosphatase , mtt assay , bone morphogenetic protein 2 , chemistry , oncogene , pharmacology , in vitro , bone remodeling , western blot , molecular medicine , medicine , endocrinology , cell cycle , apoptosis , biochemistry , enzyme , gene

The dihydropyridine-type calcium channel blocker, benidipine (BD) has been widely used in hypertension therapy. Previous studies have demonstrated that BD has a positive effect on bone metabolism. Inspired by this promoting phenomenon, the present study investigated the effects of BD on osteoblasts in vitro . Experiments were designed and performed, including an MTT assay, reverse transcription-polymerase chain reaction, western blot analysis, alkaline phosphatase activity measurements and alizarin red S staining. The results demonstrated that BD promoted osteoblast proliferation and osteogenic differentiation at concentrations from 1×10 -6 to 1×10 -9 M by upregulating Runx2, BMP2 and OCN gene expression levels. Overall, BD at appropriate concentrations has been demonstrated to have positive effects on osteoblast function in addition to its conventional clinical usage.

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