Open AccessEffects of the antihypertensive drug benidipine on osteoblast function in vitro
Author(s) -
Baixiang Wang,
Minghong Bi,
Zhen Zhu,
Lei Wu,
Jingyun Wang
Publication year - 2014
Publication title -
experimental and therapeutic medicine
Language(s) - English
Resource type - Journals
eISSN - 1792-1015
pISSN - 1792-0981
DOI - 10.3892/etm.2014.1475
Subject(s) - runx2 , osteoblast , alkaline phosphatase , mtt assay , bone morphogenetic protein 2 , chemistry , oncogene , pharmacology , in vitro , bone remodeling , western blot , molecular medicine , medicine , endocrinology , cell cycle , apoptosis , biochemistry , enzyme , gene
The dihydropyridine-type calcium channel blocker, benidipine (BD) has been widely used in hypertension therapy. Previous studies have demonstrated that BD has a positive effect on bone metabolism. Inspired by this promoting phenomenon, the present study investigated the effects of BD on osteoblasts in vitro . Experiments were designed and performed, including an MTT assay, reverse transcription-polymerase chain reaction, western blot analysis, alkaline phosphatase activity measurements and alizarin red S staining. The results demonstrated that BD promoted osteoblast proliferation and osteogenic differentiation at concentrations from 1×10 -6 to 1×10 -9 M by upregulating Runx2, BMP2 and OCN gene expression levels. Overall, BD at appropriate concentrations has been demonstrated to have positive effects on osteoblast function in addition to its conventional clinical usage.