Open AccessmicroRNA-based cancer cell reprogramming technology
Author(s) -
Shimpei Nishikawa,
Hideshi Ishii,
Naotsugu Haraguchi,
Yoshihiro Kano,
Takahito Fukusumi,
Katsuya Ohta,
Miyuki Ozaki,
Dyah Laksmi Dewi,
Daisuke Sakai,
Taroh Satoh,
Hiroaki Nagano,
Yuichiro Doki,
Masaki Mori
Publication year - 2012
Publication title -
experimental and therapeutic medicine
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1792-1015
pISSN - 1792-0981
DOI - 10.3892/etm.2012.558
Subject(s) - reprogramming , klf4 , sox2 , biology , induced pluripotent stem cell , microrna , ectopic expression , epigenetics , oncogene , regenerative medicine , somatic cell , microbiology and biotechnology , cancer , cancer cell , cancer research , cell cycle , transcription factor , cell , stem cell , embryonic stem cell , genetics , cell culture , gene
Epigenetic modifications play crucial roles in cancer initiation and development. Complete reprogramming can be achieved through the introduction of defined biological factors such as Oct4, Sox2, Klf4, and cMyc into mouse and human fibroblasts. Introduction of these transcription factors resulted in the modification of malignant phenotype behavior. Recent studies have shown that human and mouse somatic cells can be reprogrammed to become induced pluripotent stem cells using forced expression of microRNAs, which completely eliminates the need for ectopic protein expression. Considering the usefulness of RNA molecules, microRNA-based reprogramming technology may have future applications in regenerative and cancer medicine.