Open AccessDownregulation of activin‑signaling gene expression in passaged normal human dermal fibroblasts
Author(s) -
Young Il Kim,
ChanYang Lee,
Min Kyung Shin
Publication year - 2019
Publication title -
biomedical reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.607
H-Index - 25
eISSN - 2049-9442
pISSN - 2049-9434
DOI - 10.3892/br.2019.1258
Subject(s) - follistatin , activin type 2 receptors , acvr2b , smad , biology , activin receptor , transforming growth factor , signal transduction , downregulation and upregulation , messenger rna , smad2 protein , phosphorylation , endocrinology , medicine , oncogene , tgf beta signaling pathway , microbiology and biotechnology , gene , cell cycle , genetics
Activins are members of the transforming growth factor-β (TGF-β) superfamily and play important roles in proliferation, differentiation, and apoptosis of various target cells. We investigated changes of activin, activin receptor (ActR), and Smad-signaling gene expression with increasing passage number in normal human dermal fibroblasts. The expression of mRNA and protein was measured by reverse transcription-quantitative polymerase chain reaction and immunoblot analysis from passage numbers 5 to 15. Activin A and follistatin transcript levels increased with increasing passage number. ActR types IA, IB, IIA and IIB mRNA levels decreased at high passage number. The levels of Smad2, 3 and 4 protein decreased with increasing passage number, which also attenuated phosphorylation of Smad2 and 3 protein expression. Smad7 was enhanced with increasing passage number. These results suggest that expression of activin-signaling in aging normal human dermal fibroblasts increases activin A and follistatin, whereas ActR-Smad signaling is decreased.