Open AccessAssociation of C677T MTHFR and G20210A FII prothrombin polymorphisms with susceptibility to myocardial infarction
Author(s) -
Wiam Hmimech,
Hind Hassani Idrissi,
Bréhima Diakité,
Dalila Baghdadi,
Farah Korchi,
Rachida Habbal,
Sellama Nadifi
Publication year - 2016
Publication title -
biomedical reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.607
H-Index - 25
eISSN - 2049-9442
pISSN - 2049-9434
DOI - 10.3892/br.2016.717
Subject(s) - methylenetetrahydrofolate reductase , hindiii , genotype , heterozygote advantage , medicine , prothrombin g20210a , allele , single nucleotide polymorphism , gastroenterology , genetics , myocardial infarction , biology , restriction enzyme , gene
Myocardial infarction (MI) is a common complex pathology, localized in the main leading causes of mortality worldwide. It is the result of the interaction of genetic and environmental factors. The aim of the present study was to investigate the potential association of C677T 5,10-methylenetetrahydrofolate reductase ( MTHFR ) (rs1801133) and G20210A factor II prothrombin ( FII ) (rs1799963) polymorphisms with the susceptibility of MI. Following extraction by the standard salting-out procedure, DNA samples of 100 MI patients and 182 apparently healthy controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism using Hinf I and Hind III restriction enzymes, respectively. The results show a significant association of the G20210T FII polymorphism with the MI risk. The frequencies of the heterozygote genotype GA, homozygous mutated AA and the G20210A allele was higher among patients compared to controls (GA: 59 vs. 5.5%, P<0.001; AA: 10 vs. 0%, P=0.003; and 20210A: 39.5 vs. 2.7%, P<0.003), suggesting that this polymorphism may be a potential genetic marker for MI. No significant association was observed between the C677T MTHFR and MI occurrence, and there was more heterozygote CT in the patient group compared to the controls. As a multifactorial disease, the development of MI may be the result of numerous factors that influence synergistically its occurrence. Thus, further studies are merited to try to better assess these associations (gene-gene and gene-environment interactions).