Open AccessEffect of Different Solvent on Phytochemical Content, Tyrosinase Inhibition and Antioxidant Activities of Campolay (Pouteria campechiana kunth. [Baehni.])
Author(s) -
Sani Nurlaela Fitriansyah,
Rika Hartati,
Irda Fidrianny
Publication year - 2022
Publication title -
open access macedonian journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.288
H-Index - 17
ISSN - 1857-9655
DOI - 10.3889/oamjms.2022.8204
Subject(s) - tyrosinase , quercitrin , flavonoid , dpph , antioxidant , phytochemical , ethyl acetate , ic50 , medicine , traditional medicine , food science , chromatography , chemistry , biochemistry , in vitro , enzyme
BACKGROUND: Pouteria campechiana leaves are reported to have phenol and flavonoid compounds. Phenolic and flavonoid compounds can act as tyrosinase inhibitor and antioxidant.AIM: The purpose of this study was to compare phytochemical content, tyrosinase inhibition, antioxidant activities, and determine of marker compound from P. campechiana leaves extract with different polarities solvent.METHODS: In addition, the content of marker compound from P. campechaina leaves extract was determined by HPLC.RESULTS: The highest total phenolic content (TPC) 7.83 GAE/100 g extract, IC50 of tyrosinase 171.512 ± 1.352 and IC50 of DPPH 0.968 ± 0.008 was given by ethanolic extract (DE). Meanwhile ethyl acetate extract (DEA) had the highest of total flavonoid content 2.544 ± 0.554 QEA/100 g extract. The TPC correlated with tyrosinase inhibitory activity and antioxidant activity.CONCLUSION: Quercitrin was as marker compound from P. campechiana leaves extract, and quercitrin content in the DEA of P. campechiana leaves was 3.539%, while in the DE was 0.153%.