Open Accessvon Willebrand Factor Gene Polymorphism in Preeclampsia Pregnant at Medan, Indonesia
Author(s) -
Dewi Indah Sari Siregar,
Muhammad Fidel Ganis Siregar,
Gontar Alamsyah Siregar,
Syah Mirsya Warli
Publication year - 2021
Publication title -
open access macedonian journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.288
H-Index - 17
ISSN - 1857-9655
DOI - 10.3889/oamjms.2021.7732
Subject(s) - medicine , preeclampsia , genotype , von willebrand factor , hemostasis , agarose gel electrophoresis , microbiology and biotechnology , polymorphism (computer science) , polymerase chain reaction , platelet , gene , pregnancy , genetics , biology
BACKGROUND: von Willebrand Factor (vWF) is a large glycoprotein mediating hemostasis and thrombosis. The roles of vWF are platelets adhesion to sites of vascular damage and stabilization of coagulation factor VIII.AIM: This study aimed to analyze the polymorphism of the vWF gene on preeclampsia (PE) in pregnancy in Medan, Indonesia.MATERIALS AND METHODS: DNA was amplified using the polymerase chain reaction and was electrophoresed in agarose 2%. Electrophoresis results were detected using Gel Doc 1000 (Biorad, USA). The sequencing method was used to identify polymorphism from vWF gene.RESULTS: From 50 samples of PE patients, the g.93308C>T vWF gene polymorphism was found with the percentage of TT, CT, and CC genotypes as 50%, 42%, and 8%, respectively.CONCLUSION: The c.93308C>T vWF gene polymorphism was found in the genotype percentage of homozygous TT, and heterozygote CT was greater than wild-type CC.