Open AccessProtective Effect of Eugenol against Acetaminophen-Induced Hepatotoxicity in Human Hepatocellular Carcinoma Cells via Antioxidant, Anti-Inflammatory, and Anti-Necrotic Potency
Author(s) -
Florenly Florenly,
Liena Sugianto,
I Nyoman Ehrich Lister,
Ermi Girsang,
Chrismis Novalinda Ginting,
Ervi Afifah,
Hanna Sari Widya Kusuma,
Rizal Rizal,
Wahyu Widowati
Publication year - 2021
Publication title -
open access macedonian journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.288
H-Index - 17
ISSN - 1857-9655
DOI - 10.3889/oamjms.2021.7003
Subject(s) - eugenol , medicine , acetaminophen , pharmacology , oxidative stress , antioxidant , reactive oxygen species , apoptosis , cyp2e1 , tumor necrosis factor alpha , inflammation , hepatocellular carcinoma , liver injury , biochemistry , immunology , cytochrome p450 , chemistry , metabolism , organic chemistry
BACKGROUND: Overdoses acetaminophen (APAP) could cause acute liver failure, even though it used is for analgesics. APAP could cause hepatotoxicity due to multiple mediators of inflammation and oxidative stress. Eugenol has been reported to have anti-inflammatory and antioxidant activity but its hepatoprotective effect has not been widely reported.AIM: The purpose of this research is to know if eugenol could protect HepG2 cells from APAP.METHODS: HepG2 that induced by APAP as hepatotoxicity cells model was treated by using eugenol at 6.25 and 25 μg/mL. The protective effects of eugenol toward hepatotoxicity were evaluated by determine tumor necrosis factor-α (TNF-α) concentration, apoptotic activity, reactive oxygen species (ROS) level, also cytochrome (CYP)2E1 and GPX gene expression.RESULTS: Eugenol at 6.25 and 25 μg/mL concentration can reduce TNF-α concentration, the apoptotic, necrotic, dead cells, and ROS level. Besides it can increase the gene expression (GPX and CYP2E1). The best hepatoprotective effect was found when using the eugenol at 25 μg/mL.CONCLUSION: Therefore, eugenol can be used to protect HepG2 cells against APAP.