Open AccessPreimplantation Genetic Testing of Aneuploidy by Next Generation Sequencing: Association of Maternal Age and Chromosomal Abnormalities of Blastocyst
Author(s) -
Truong Tien Dang,
Thi Mui Phung,
Hoang M. Le,
Thi-Bich-Van Nguyen,
Thi Sim Nguyen,
Thi Lien Huong Nguyen,
Vu Thi Nga,
DinhToi Chu,
Van Luong Hoang,
Duy Nguyen
Publication year - 2019
Publication title -
open access macedonian journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.288
H-Index - 17
ISSN - 1857-9655
DOI - 10.3889/oamjms.2019.875
Subject(s) - aneuploidy , blastocyst , advanced maternal age , andrology , chromosome , embryo , medicine , chromosome abnormality , in vitro fertilisation , karyotype , biology , genetics , pregnancy , embryogenesis , gene , fetus
BACKGROUND: Aneuploidy is a major cause of miscarriages and implantation failure. Preimplantation genetic testing for aneuploidy (PGT-A) by Next Generation Sequencing (NGS) is able to detect of the numeral and structural chromosomal abnormalities of embryos in vitro fertilization (IVF).
AIM: This study was aimed to assess the relationship between maternal age and chromosomal abnormalities NGS technology.
METHODS: 603 human trophectoderm (TE) biopsied samples were tested by Veriseq kit of Illumina. The relation of marternal age and chromosomal abnormality of blastocyst embryo was evaluated.
RESULTS: Among the 603 TE samples, 247 samples (42.73%) presented as chromosomal abnormalities. The abnormalities occurred to almost chromosomes, and the most popular aneuploidy observed is 22. Aneuploidy rate from 0.87% in chromosome 11 to 6.06% in chromosome 22. The rate of abnormal chromosome increased dramatically in group of mother's ages over 37 (54.17%) comparing to group of mother's ages less than 37 (38.05%) (p < 0.000). The Abnormal chromosome and maternal age has a positive correlation with r = 0.4783 (p<0.0001).
CONCLUSION: These results showed high rate abnormal chromosome and correlated with advanced maternal age of blastocyst embryos.