Open AccessDEVELOPMENT OF A GFP‐BASED BIOSENSOR FOR DETECTING THE BIOAVAILABILITY AND BIODEGRADATION OF POLYCHLORINATED BIPHENYLS (PCBS)
Author(s) -
Xuemei Liu,
Kieran J. Germaine,
David Ryan,
David N. Dowling
Publication year - 2007
Publication title -
journal of environmental engineering and landscape management
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.514
H-Index - 28
eISSN - 1822-4199
pISSN - 1648-6897
DOI - 10.3846/16486897.2007.9636939
Subject(s) - biosensor , inducer , benzoic acid , biodegradation , chemistry , bioavailability , polychlorinated biphenyl , green fluorescent protein , in vitro , biphenyl , fluorescence , environmental chemistry , chromatography , biochemistry , organic chemistry , biology , gene , physics , quantum mechanics , bioinformatics
Two whole-cell biosensors were constructed to detect the in situ biodegradation of polychlorinated biphenyl by chromosomal insertion of a mini‐Tn5‐Kmr‐Pm::gfp[mut3]‐T0‐T1 construct into P. fluorescens. In vitro tests showed that the expression of the Pm promoter depended on the growth phase of the biosensors and the concentration of chemical inducers; chlorinated benzoic acid derivatives. A linear relationship between the fluorescent intensity and the log10 concentration of the inducer was observed. One biosensor (F113L::1180gfp) had the ability to degrade PCBs to relevant chlorobenzoic acid derivatives and to induce expression of Gfp. The second biosensor (F113gfp), which cannot degrade PCBs, shows fluorescence after induction by chloro‐benzoic acid derivatives. By using these two biosensors, PCB degradation could be detected in vitro and in soil.