Open AccessThe Role of a Combination of Autogenous Fresh Bone Marrow and Omental Free Graft in the Cervical Esophageal Wound Healing in Dogs
Author(s) -
Omid Azari,
Mohammad Mahdi Molaei,
Reza Kheirandish,
Sara Hamzeh Aliabad
Publication year - 2010
Publication title -
american journal of animal and veterinary sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.174
H-Index - 15
eISSN - 1557-4563
pISSN - 1557-4555
DOI - 10.3844/ajavsp.2010.202.207
Subject(s) - medicine , surgery , wound healing , esophagus , fibrous joint , bone marrow , h&e stain , pathology , staining
Problem statement: Esophageal surgery is associated with greater wound healing problems
than surgery on other portion of the alimentary tract. Omentum and fresh bone marrow are rich source
of angiogenic factors that promote the growth of blood vessels into wound tissue. The aim of this study
was evaluation of the effects of a combination of autogenous fresh bone marrow and omental free graft
in esophageal wound healing. Approach: This study was carried out on 8 male adult dogs, assigned
into two equal treatment and control groups. All dogs underwent cervical esophagotomy and
laparotomy operations, also bone marrow aspiratin, simultaneously. A 3 cm linear full thickness
incision was made on the cervical esophagus and then sutured. In treatment group, A small piece of
omentum was obtained from abdominal cavity and secured on esophageal suture line and then 1 mL of
fresh bone marrow was injected into the omental graft and wound edge. In the control group, the suture
line was left without any treatment and the harvested omentum and aspirated bone marrow were
disposed. During this study, the dogs were examined clinically and they were euthanized 14 days after
operation. The surgical site of esophagus was assessed macroscopically and then histopathologic samples
were taken from the repaired tissue of esophageal wall. The samples were stained by hematoxylin-eosin
and masson-trichorome. Results: The clinical evaluation of present study revealed that all dogs were
healthy with no major postoperative complications. There was no significant difference based on
macroscopic examination in surgical site of esophagus between the two groups. The microscopic study
revealed that inflammatory cells, angiogenesis and granulation tissue formation in wound bed of the
treatment group were significantly more than the control group. Conclusion: According to the results of
this study, application of a combination of autogenously fresh bone marrow and free omental graft can
improve wound healing process in esophageal incision wounds