Open AccessAn Active CACTA‐Family Transposable Element is Responsible for Flower Variegation in Wild Soybean Glycine soja
Author(s) -
Takahashi Ryoji,
Morita Yasumasa,
Nakayama Masayoshi,
Kanazawa Akira,
Abe Jun
Publication year - 2012
Publication title -
the plant genome
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 41
ISSN - 1940-3372
DOI - 10.3835/plantgenome2011.11.0028
Subject(s) - biology , glycine soja , transposable element , genetics , locus (genetics) , exon , gene , inverted repeat , variegation (histology) , ethyl methanesulfonate , mutant , glycine , amino acid , genome
A plant producing flowers with purple and white variegation was discovered in an accession of Glycine soja Siebold & Zucc. that was introduced from Russia. The mutant line was designated as B00146‐m. Lines with white flowers (B00146‐w) and purple flowers (B00146‐r) were developed from the progeny of B00146‐m. The flower color was controlled by the W1 locus encoding a flavonoid 3′5′‐hydroxylase (F3′5′H). The allele for variegated flowers was designated as w1‐m . The gene symbol was approved by the Soybean Genetics Committee. Polymerase chain reaction (PCR) suggested that a DNA fragment with a molecular size of ∼3.9 kb was inserted in the first exon of the F3 ′ 5 ′ H gene in B00146‐m whereas such insertion was not observed in B00146‐w and B00146‐r. These results suggested that an active mobile element was inserted in the first exon and was responsible for flower variegation. The inserted fragment was identified as a 3883 bp long CACTA‐family transposable element and it was designated as Tgs1 . Similarity of overall sequence and terminal inverted repeats suggested that Tgs1 and the soybean lectin gene transposable element Tgm1 make up a subgroup. Frequency of germinal reversion was low probably due to the integration into an exon. Tgs1 had a truncated version of the transposase gene and may be a nonautonomous element.