Open AccessFine Mapping of the SCN Resistance Locus rhg1‐b from PI 88788
Author(s) -
Kim Myungsik,
Hyten David L.,
Bent Andrew F.,
Diers Brian W.
Publication year - 2010
Publication title -
the plant genome
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 41
ISSN - 1940-3372
DOI - 10.3835/plantgenome2010.02.0001
Subject(s) - soybean cyst nematode , locus (genetics) , heterodera , genetics , biology , gene , allele , nematode , ecology
Soybean cyst nematode (SCN) ( Heterodera glycines Ichinohe) is the most economically damaging soybean [ Glycine max (L.) Merr.] pest in the USA and genetic resistance is a key component for its control. Although SCN resistance is quantitative, the rhg1 locus on chromosome 18 (formerly known as Linkage Group G) confers a high level of resistance. The objective of this study was to fine‐map the rhg1‐b allele that is derived from plant introduction (PI) 88788. F 2 and F 3 plants and F 3:4 lines from crosses between SCN resistant and susceptible genotypes were tested with genetic markers to identify recombination events close to rhg1‐b . Lines developed from these recombinant plants were then tested for resistance to the SCN isolate PA3, which originally had an HG type 0 phenotype, and with genetic markers. Analysis of lines carrying key recombination events positioned rhg1‐b between the simple sequence repeat (SSR) markers BARCSOYSSR_18_0090 and BARCSOYSSR_18_0094. This places rhg1‐b to a 67‐kb region of the ‘Williams 82’ genome sequence. The receptor‐like kinase gene that has been previously identified as a candidate for the ‘Peking’‐derived SCN resistant rhg1 gene is adjacent to, but outside of, the rhg1‐b interval defined in the present study.