Open AccessMolecular Cloning and Linkage Mapping of Cryptochrome Multigene Family in Soybean
Author(s) -
Matsumura Hisakazu,
Kitajima Hiroshi,
Akada Shinji,
Abe Jun,
Minaka Nobuhiro,
Takahashi Ryoji
Publication year - 2009
Publication title -
the plant genome
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 41
ISSN - 1940-3372
DOI - 10.3835/plantgenome.2009.06.0018
Subject(s) - cryptochrome , biology , genetics , gene , amplicon , exon , gene family , intron , microbiology and biotechnology , polymerase chain reaction , genome , circadian clock
The cryptochromes are a family of blue light photoreceptors that play important roles in the controls of plant development. Seven full‐length cryptochrome cDNAs (GmCRY1a, GmCRY1b, GmCRY1c, GmCRY1d, GmCRY2a, GmCRY2b, and GmCRY2c) were isolated by cDNA library screening and reverse transcriptase–polymerase chain reaction from ‘Williams’ soybean [ Glycine max (L.) Merr.], indicating that soybean cryptochrome genes comprise a multigene family. They had homologies ranging from 60 to 89% with CRY1 and CRY2 genes of Arabidopsis and pea ( Pisum sativum L.). Two types of transcripts were isolated in GmCRY1b, GmCRY1c, GmCRY1d, and GmCRY2a. One type was derived from four exons, whereas the other type was derived from five exons. Occurrence of the former transcript could be explained by retention of the fourth intron, suggesting existence of alternative splicing. Gene sequences were compared between a soybean line, Tokei 780, and an accession of soybean wild relative, Glycine soja , Hidaka 4. Based on a 10‐bp indel, an amplicon length polymorphism (ALP) marker was designed for mapping of GmCRY2b. For mapping of the other cryptochrome genes, derived cleaved amplified polymorphic sequence (dCAPS) markers were constructed. The cryptochrome genes were individually assigned to different molecular linkage groups (MLG) (GmCRY1a: MLG C1; GmCRY1b: C2; GmCRY1c: B2; GmCRY1d: F; GmCRT2a: O; GmCRY2b: D1b; GmCRY2c: I). The distribution of cryptochrome genes that was deduced from the soybean genome database was consistent with mapping results.