Open AccessFluorescent Method for Observing Intravascular Bonghan Duct
Author(s) -
Byung-Cheon Lee,
Ku Youn Baik,
Hyeon-Min Johng,
Baekkyoung Sung,
Kyung Soon Soh,
Dae-In Kang,
Kwang–Sup Soh
Publication year - 2005
Publication title -
yag'cim haghoeji
Language(s) - English
Resource type - Journals
ISSN - 1226-4849
DOI - 10.3831/kpi.2005.8.3.005
Subject(s) - fluorescence , optics , physics
Observation of intra-vascular threadlike structures in the blood vessels of rats is reported with the images by differential interference contrast microscope, and fluorescence inverted microscope of the acridine-orange stained samples. The confocal microscope image and the hematoxylin-eosin staining revealed the distinctive pattern of nuclei distribution that clearly discerned the threadlike structure from fibrin, capillary, small venule, arteriole, or lymph vessel. Physiological function of the intra-vascular thread in connection with acupuncture is discussed. Especially, this threadlike duct can be a circulation path for herb-liquid flow, which may provide the scientific mechanism for therapeutic effect of herbal acupuncture