Open AccessImpact of Intrauterine Growth Restriction Diseases on The Umbilical Cord Blood CD34+ Cell Counts
Author(s) -
Ekrem Ünal,
Şerife Erdem,
Ayten Biçer,
Fatma Zehra Okuş,
Alper Özcan,
Zehra Büşra Azizoğlu,
Ahmet Eken,
Semih Uludağ,
Mehmet Serdar Kütük,
Musa Karakükçü,
Ekrem Ünal
Publication year - 2020
Language(s) - English
DOI - 10.38092/jpa-2020-878330
Subject(s) - umbilical cord , cord blood , cd34 , andrology , peripheral blood mononuclear cell , placenta cord banking , flow cytometry , medicine , progenitor cell , immunology , haematopoiesis , gestational age , placenta , stem cell , biology , fetus , pregnancy , biochemistry , genetics , in vitro
Different diseases in obstetrics and gynecology can affect the number of CD34+ cells inthe umbilical cord blood.Objectives: This study aimed to evaluate the effect of Gestational Diabetes Mellitus (GDM), GestationalHypertension (GHT) and Morbidly Adherent Placenta (MAP) on the content of CD34+ cells of umbilicalcord blood and to compare the effectiveness of Sysmex XN20 analyzer to the flow cytometry method,which is the gold standard in CD34+ cells.Materials abd Methods: The umbilical cord blood (15 ml) was collected after the birth of the newborns.Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque Plus. The cells were stainedwith Procount ™ Progenitor Cell Count Kit with PE Labeled monoclonal anti-CD34+ antibody thenanalyzed with Flow Cytometry or Sysmex XN20 without staining, to identify CD34+ cells named ashematopoietic progenitor cells (HPC), respectively.Results: Flow cytometric evaluation revealed a significantly elevated (p<0.05) number of cord bloodCD34+ cells in GDM and GHT groups compared with healthy controls. MAP patients had comparableCD34+ cells compared with healthy controls. A significant increase in lymphocyte counts was alsoobserved in GDM and GHT groups compared with healthy controls. Sysmex analysis however onlyrevealed an increase in lymphocyte numbers in GHT but picked no differences across groups in HPC.Correlation between Sysmex and flow cytometry results was weak in control, GHT, GDM and MAPgroups r: 0570/p<0.01, r: 0.5727/p: 0.0708, r:0.2149/p: 0.4779, r: 0.111/p: 0.779, respectively.Conclusions: CD34+ cells were significantly higher in the GHT and GDM groups compared with healthycontrol cord blood. The correlations between Flow cytometry and Sysmex were not strong.