Open AccessOxidative stress and low molecular weight antioxidants in triticale shoots under chloride salinization
Author(s) -
V. V. Ivanishchev
Publication year - 2020
Publication title -
butlerovskie soobŝeniâ
Language(s) - English
Resource type - Journals
ISSN - 2074-0212
DOI - 10.37952/roi-jbc-01/20-62-6-125
Subject(s) - ascorbic acid , chemistry , hydrogen peroxide , proline , triticale , antioxidant , lipid peroxidation , glutathione , biochemistry , sodium ascorbate , food science , sodium , botany , amino acid , organic chemistry , biology , enzyme
We studied the alterations in oxidative stress indicators (hydrogen peroxide, superoxide radical, lipid peroxidation – LPO) and the alterations in the content of low molecular weight metabolites (ascorbic acid, glutathione, proline, chlorophyll and carotenoids) in the shoots of triticale (Triticosecale) under short-term (0-96 h) sodium chloride stress (120 mM) with statistical methods: principal component analysis (PCA) and cluster analysis. An analysis of the alterations in oxidative stress indicators allowed us to calculate the correlation coefficients for the pairs: peroxide – superoxide (0.52), peroxide – LPO (0.62), superoxide – LPO (0.23). The inclusion in the analysis of data on alterations in the content of low molecular weight antioxidants showed that the PCA method forms three main groups for all the studied characteristics: (1) LPO and hydrogen peroxide, (2) chlorophyll and carotenoids, (3) glutathione and ascorbate. The correlation coefficients were calculated for pairs: ascorbate – glutathione (0.71), ascorbate – proline (0.81), glutathione – proline (0.28). Such a value of the coefficient of the first pair suggests that ascorbic acid also performs numerous other functions, in addition to participating in the ascorbate-glutathione cycle. The high correlation between ascorbate and proline can be explained by the similar nature of alterations in their content in triticale shoots under conditions of short-term sodium chloride stress. Negative correlation coefficients in pairs of hydrogen peroxide – chlorophyll (-0.73), peroxide – carotenoids (-0.75), ascorbic acid – LPO (-0.70), LPO – proline (-0.69) give reason to talk about the possible protective role of photosynthesis pigments from accumulating hydrogen peroxide, as well as the potential role of ascorbic acid and proline in protecting membranes from lipid peroxidation. The application of the cluster analysis method showed that first and second order clusters between ascorbate, proline and glutathione reflect their known antioxidant role. The results obtained may also indicate that pigments have a much lower protective function.