Open AccessA Mouse Model for Chronic Pancreatitis via Bile Duct TNBS Infusion
Author(s) -
Wenyu Gou,
Lindsay Swaby,
Amber Wolfe,
William Lancaster,
Katherine A. Morgan,
Hongjun Wang
Publication year - 2021
Publication title -
journal of visualized experiments
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 91
ISSN - 1940-087X
DOI - 10.3791/62080
Subject(s) - pancreatitis , medicine , pancreatic duct , duodenum , pancreas , abdominal pain , bile duct , gastroenterology , ampulla of vater , common bile duct , ampulla , pancreatic disease , drug , acute pancreatitis , atrophy , pharmacology , carcinoma
Chronic pancreatitis (CP) is a complex disease involving pancreatic inflammation and fibrosis, glandular atrophy, abdominal pain and other symptoms. Several rodent models have been developed to study CP, of which the bile duct 2,4,6 -trinitrobenzene sulfonic acid (TNBS) infusion model replicates the features of neuropathic pain seen in CP. However, bile duct drug infusion in mice is technically challenging. This protocol demonstrates the procedure of bile duct TNBS infusion for generation of a CP mouse model. TNBS was infused into the pancreas through the ampulla of Vater in the duodenum. This protocol optimized drug volume, surgical techniques, and drug handling during the procedure. TNBS-treated mice showed features of CP as reflected by bodyweight and pancreas weight reductions, changes in pain-associated behaviors, and abnormal pancreatic morphology. With these improvements, mortality associated with TNBS injection was minimal. This procedure is not only critical in generating pancreatic disease models but is also useful in local pancreatic drug delivery.