Open AccessInduction of Diffuse Axonal Brain Injury in Rats Based on Rotational Acceleration
Author(s) -
Dmitry Frank,
Israel Melamed,
Benjamin F. Gruenbaum,
Julia Grinshpun,
Ruslan Kuts,
Rachel Shvartsur,
Abed N. Azab,
Mohamad H Assadi,
Max Vinokur,
Matthew Boyko
Publication year - 2020
Publication title -
journal of visualized experiments
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 91
ISSN - 1940-087X
DOI - 10.3791/61198
Subject(s) - diffuse axonal injury , traumatic brain injury , rodent model , blast injury , medicine , white matter , traumatic injury , neuroscience , poison control , surgery , magnetic resonance imaging , biology , psychiatry , environmental health , radiology
Traumatic brain injury (TBI) is a major cause of death and disability. Diffuse axonal injury (DAI) is the predominant mechanism of injury in a large percentage of TBI patients requiring hospitalization. DAI involves widespread axonal damage from shaking, rotation or blast injury, leading to rapid axonal stretch injury and secondary axonal changes that are associated with a long-lasting impact on functional recovery. Historically, experimental models of DAI without focal injury have been difficult to design. Here we validate a simple, reproducible and reliable rodent model of DAI that causes widespread white matter damage without skull fractures or contusions.