Open AccessInducement and Evaluation of a Murine Model of Experimental Myopia
Author(s) -
Xiaoyan Jiang,
Michio Ohta,
Shigeto Ikeda,
Hiromitsu Kunimi,
Kiwako Mori,
Hidemasa Torii
Publication year - 2019
Publication title -
journal of visualized experiments
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 91
ISSN - 1940-087X
DOI - 10.3791/58822
Subject(s) - extant taxon , lens (geology) , optometry , protocol (science) , computer science , refraction , refractive error , ophthalmology , medicine , artificial intelligence , optics , biology , pathology , physics , eye disease , evolutionary biology , alternative medicine
Murine model of myopia can be a powerful tool for myopia research because of the comparatively easy genetic manipulation. One way to induce myopia in animals is to put clear minus lenses in front of eyes for weeks (lens-induced myopia, LIM). However, extant protocols for inducement and evaluation vary from laboratory to laboratory. Here, we described a highly practical and reproducible method to induce LIM in mice using newly designed eyeglasses. The method fixes the lens stably in front of the mouse eye while allows the lens to be taken off for cleaning or topical drug administration. The phenotype is robust and efficient, and the variance is small. The method described here can be applied to mice right after weaning which extends the possible duration for experiments. We also gave technical advises for achieving reproducible results in refraction and axial length measurements. We hope the step-by-step protocol described here and the detailed article can help researchers perform myopia experiments with myopia more smoothly and make the data comparable across laboratories.