Open AccessTotal IgE assessment in newborn umbilical cord blood
Author(s) -
Mariana Vieru,
Anca A. Simionescu
Publication year - 2021
Publication title -
romanian journal of medical practice
Language(s) - English
Resource type - Journals
eISSN - 2069-6108
pISSN - 1842-8258
DOI - 10.37897/rjmp.2021.s4.4
Subject(s) - umbilical cord , immunoglobulin e , immunoassay , medicine , immunology , cord blood , biomarker , blood sampling , blood collection , allergy , antibody , chemistry , biochemistry , emergency medicine
In this minireview we present important data on umbilical cord blood IgE antibodies in neonates and total IgE as a predictive biomarker for the development of allergen sensitization and atopic diseases later in life. Discussions regarding the methods for determining total IgE in serum or plasma from cord blood samples are focused on the main immunoassays used in different studies and clinical practice. The fluorescence enzyme immunoassay with anti-IgE covalently coupled to a capsulated cellulose polymer solid-phase is nowadays currently used to measure total IgE in human serum or plasma. The umbilical cord blood total IgE levels are quantitatively determined by using its low range assay. Specific and practical aspects regarding cord blood sampling, including specimen collection from the umbilical cord vessels and careful preparation of serum and plasma, alongside with knowledge of the principles of the immune methods used are important to avoid preanalytical and analytical errors and for obtaining accurate results of this risk biomarker for allergy.