Open AccessDEVELOPMENT AND VALIDATION OF NEW METHOD FOR THE SIMULTANEOUS ESTIMATION OF CEFTRIAXONE AND TAZOBACTAM USING RP-HPLC
Author(s) -
Y. Rajendra Prasad,
Sridevi Ranjitha Karanam
Publication year - 2022
Publication title -
ymer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.103
H-Index - 5
ISSN - 0044-0477
DOI - 10.37896/ymer21.03/06
Subject(s) - ceftriaxone , tazobactam , chromatography , high performance liquid chromatography , chemistry , antibiotics , biochemistry , antibiotic resistance , imipenem
The aim of this study is to create and validate a fast, easy-to-use, affordable, sensitive, and accurate method for measuring Ceftriaxone and Tazobactam in bulk and pharmaceutical products using Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC). Waters X-Terra RP-18 column was used with a running phase composed of water: acetonitrile (40:60 v/v) at a flow rate of 1.0 ml/min. UV detection was used at a wavelength of 230 nm. Tazobactam and Ceftriaxone correlation coefficients were found to be 0.999 over a concentration range of 1.25-18.75 µg/ml and 10-150 µg/ml, respectively. Tazobactam and Ceftriaxone have respective retention times of 3.131 and 2.648 minutes. The run time for separating Tazobactam and Ceftriaxone peaks was 5 minutes. The validation results agreed with what's acceptable and had good limits. This method, proposed as a regular analysis and quality control tool for medications that contain these active drugs either individually or in combination, was evident to be a suitable one.