NEW METHOD FOR THE SIMULTANEOUS ESTIMATION OF SIPONIMOD AND PONESIMOD BY USING ULTRA PERFORMANCE LIQUID CHROMATOGRAPHIC SYSTEM IN ACTIVE PHARMACEUTICAL INGREDIENT FORM

The aim of this study is to create and validate a fast, easy-to-use, affordable, sensitive, and accurate method for measuring Siponimod and Ponesimod in bulk and pharmaceutical products using Reversed-Phase Ultra-Performance Liquid Chromatography (RP-UPLC). A Luna C18 column was used with a running phase composed of 0.1% trifluoroacetic acid: acetonitrile (30:70 v/v) at a flow rate of 1.0 ml/min. UV detection was used at a wavelength of 230 nm. Ponesimod and Siponimod correlation coefficients were found to be 0.999 over a concentration range of 5-75 µg/ml and 0.5-7.5 µg/ml, respectively. Ponesimod and Siponimod have respective retention times of 1.751 and 3.203 minutes. The run time for separating Ponesimod and Siponimod peaks was 5 minutes. The validation results agreed with what's acceptable and had good limits. This method, proposed as a regular analysis and quality control tool for medications that contain these active drugs either individually or in combination, was evident to be a suitable one.