Open AccessANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF CAPMATINIB IN BULK AND TABLET DOSAGE FORM BY RP-HPLC METHOD
Author(s) -
M Sivaganesh,
AUTHOR_ID
Publication year - 2022
Publication title -
ymer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.103
H-Index - 5
ISSN - 0044-0477
DOI - 10.37896/ymer21.01/56
Subject(s) - chromatography , dosage form , volumetric flow rate , high performance liquid chromatography , phosphate buffered saline , particle size , methanol , linearity , analytical chemistry (journal) , chemistry , volume (thermodynamics) , materials science , retention time , physics , organic chemistry , quantum mechanics
An HPLC method was developed and validated for the estimation of Capmatinib in bulk and pharmaceutical dosage form. The chromatographic system was equipped Agilent column 150 mm x 4.6 mm internal diameter with 5 micron particle size column and UV detector set at 218nm, in conjunction with a mobile phase of Methanol and phosphate buffer PH 5 in the ratio of (65:35) at a flow rate of 0.5 ml/min. The retention time of capmatinib was found to be 4.3 minute. The separation was performed at ambient temperature. The injection volume was 10μl. Linearity in concentration range of 10-50μg mL with regression 0.999. The Percentage recoveries were found in the range of 101.4– 101.8%. The proposed method was validated in accordance with ICH parameters the method is precise, accurate, selective and rapid.