Open AccessISOLASI Actinomycetes ENDOFIT DARI TANAMAN AKAR WANGI (Vetiveria zizanioides) DAN UJI AKTIVITAS SENYAWA ANTIBAKTERI TERHADAP Staphylococcus aureus DAN Escherichia coli
Author(s) -
Elsie,
Nofripa Herlina,
Ruli Tias Putri
Publication year - 2018
Publication title -
photon: jurnal sain dan kesehatan/photon : jurnal sain dan kesehatan
Language(s) - English
Resource type - Journals
eISSN - 2579-5953
pISSN - 2087-393X
DOI - 10.37859/jp.v8i2.742
Subject(s) - staphylococcus aureus , bacteria , escherichia coli , antibacterial activity , microbiology and biotechnology , biology , agar diffusion test , minimum inhibitory concentration , antimicrobial , biochemistry , genetics , gene
Actinomycetes endophytes are bacteria that live in plant tissues and are known as secondary metabolites that have activity as an antibacterial. This study was conducted to isolate Actinomycetes endophytes from vetiver plant tissue (Vetiveria zizanioides) and test for the activity of antibacterial compounds againstStaphylococcus aureus and Escherichia coli by using diffusion method. Actinomycetes endophytes successfully isolated were isolates of RTP-1, RTP-2 and RTP-3. The results of antibacterial compound activity test showed that the three Actinomycetes endophytic isolates were able to inhibit the growth of S. aureus and E. coli bacteria. The best inhibitory ability against E. coli bacteria with a drag zone diameter of 20 mm while the largest inhibitory zone diameter against S. aureus bacteria was 9 mm.