Open AccessEvaluation of Aspergillus Galactomannan Lateral Flow Assay on Serum and Bronchoalveolar Lavage Specimens
Author(s) -
Gabrijela Perše,
Ivana Mareković,
Sanja Pleško,
Violeta Rezo Vranješ,
Marija Jandrlić
Publication year - 2022
Publication title -
infektološki glasnik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.105
H-Index - 7
eISSN - 1848-7769
pISSN - 1331-2820
DOI - 10.37797/ig.41.2.1
Subject(s) - galactomannan , bronchoalveolar lavage , medicine , aspergillosis , aspergillus , gastroenterology , immunology , microbiology and biotechnology , biology , lung
Background : Detection of biomarkers, such as galactomannan (GM),has proven to be of great significance in early recognition of invasiveaspergillosis (IA). The aim of our study was to evaluate the lateral flow assay(LFA) for the detection of GM on serum and bronchoalveolar lavage (BAL) samplespreviously proven positive by enzyme-linkedimmunosorbent assay (ELISA). Methods: The study was performed on serum and BAL samples obtained from patientswith suspected IA in the period from February 2019 to January 2020, which werepreviously GM positive by ELISA (Platelia Aspergillus Ag, Biorad, Hercules, USA). Samples werethen tested by LFA ( Aspergillus Galactomannan LFA, IMMY, Oklahoma, USA) with test line intensity visually readas 1+, 2+, 3+, or 4+. Results: A total of 45 GM ELISA positive serum and/or BAL samples were obtainedfrom 41 patients; 25 (55,6 %) were BAL and 20 (44,4 %) serum samples. LFAshowed a positive result in 39 out of 45 (86,7%) GM ELISA positive samples;22/25 (88.0 %) BAL samples and 17/20 (85.0 %) serum samples tested positive. InBAL samples, low intensity test line of 1+ was significantly more frequent inGM ELISA positive samples with optical density index (ODI) < 1.0 (p=0.0002).Three serum samples with high GM ELISA ODI (>4.0) had low intensity line of1+ when tested with LFA. Conclusions: Results obtained by LFA are comparable to GMELISA. Since low intensity lines werefound in serum samples with high ODI, this potentially makes BAL a superiorsample for LFA, at least when visual and not automated reading is done.