Open AccessDEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR CONDENSED TANNIN EXTRACTS OBTAINED FROM THE BARK OF FOUR TREE SPECIES USING HPLC
Author(s) -
Faustino Ruiz Aquino,
Rossy Feria Reyes,
José Guadalupe Rutiaga-Quiñones,
Wenceslao Santiago-García,
Mario Ernesto Suárez Mota,
Héctor Hugo Esquivel Reyes
Publication year - 2021
Publication title -
wood research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.371
H-Index - 18
eISSN - 2729-8906
pISSN - 1336-4561
DOI - 10.37763/wr.1336-4561/66.2.171182
Subject(s) - bark (sound) , proanthocyanidin , tannin , fourier transform infrared spectroscopy , high performance liquid chromatography , chemistry , condensed tannin , chromatography , extraction (chemistry) , catechin , botany , organic chemistry , polyphenol , biology , food science , chemical engineering , ecology , engineering , antioxidant
Herein, we evaluated the content of condensed tannins present in the bark of four tree species that are Quercus laurina Humb. & Bonpl., Quercus crassifolia Humb. & Bonpl., Arbutus xalapensis Kunth, and Prunus serotina Ehrn. An analytical method using high-performance liquid chromatography (HPLC) for condensed tannin extracts was developed and validated. Also,the aqueous extracts were analyzed by Fourier transform infrared spectroscopy (FTIR). Based on the Stiasny number, A. xalapensis, and Q. laurina represent an important source of condensed tannins, which may be subject to exploitation. Using infrared spectroscopy, it was observed that tannins do not show an important signal of carbonyl groups (aromatic esters) with respectto high purity catechin. Furthermore, the band of the hydroxyl group is less pronouncedin tannins, because various hydroxyl are interacting with each other. However, it can be seen that the method of extraction of wood tannins developed in this work, presents satisfactory results.