Open AccessDevelopment of method for determining antioxidant activity of extracts based on deep eutectic solvents.
Author(s) -
O. I. Paukshta,
Nikita Tsvetov,
V. I. Petrova
Publication year - 2020
Publication title -
trudy kolʹskogo naučnogo centra ran
Language(s) - English
Resource type - Journals
ISSN - 2307-5252
DOI - 10.37614/2307-5252.2020.3.4.031
Subject(s) - choline chloride , acetone , antioxidant , chemistry , eutectic system , chloride , chromatography , organic chemistry , alloy
Antioxidant activity is an important characteristic of plant extracts. One of the methods to determine it, is the phosphomolybdate method, which is based on the formation of a water-soluble colored complex and the spectrophotometric determination of its concentration. Recently, methods for obtaining plant extracts using deep eutectic solvents (DES), consisting of two or more substances, have been actively developed. Many DES contain choline chloride. The presence of choline chloride makes it difficult to determine the antioxidant activity of DES-based extracts by the phosphomolybdate method since an insoluble complex is formed during the reaction. The aim of this work is to modify the phosphomolybdate method for determining the antioxidant activity of DES-based extracts by adding acetone to the mixture to transfer the resulting complex into solution.