Open AccessKaryotype and plasmotype characteristics in wheat-rye hybrids with the different structural organization of the nuclear genome
Author(s) -
Elena А. Sycheva,
A. V. Sokoluk,
M. E. Vasilevskaya,
L. A. Solovey,
Е. Б. Бондаревич,
O. M. Lyusikov,
Н. И. Дубовец
Publication year - 2021
Publication title -
journal of native and alien plant studies
Language(s) - English
Resource type - Journals
ISSN - 2707-3114
DOI - 10.37555/2707-3114.1.2021.247730
Subject(s) - biology , triticale , hybrid , introgression , ploidy , karyotype , genome , genetics , chromosome , nuclear dna , nuclear gene , chromosomal translocation , chloroplast dna , differential staining , mitochondrial dna , gene , botany , staining
Aim. Genome structure analysis and plasmotype identification in wheat-rye hybrids of various types (triti- cale, secalotriticum) and ploidy level. Mcth0ds. Cytological and molecular-genetic analysis. Rcsults. The karyotype and plasmotype analysis was carried out in 11 stable lines of secondary recombinant hexaploid triticale with the introgression of D-genome chromosomes of the wheat (A/B/DRR, 2n = 6x = 42), 14 stable and highly productive secalotriticum lines of F6–16 generations (Secalotriticum, S/RRAABB, 2n = 6x = 42), 9 stable lines of tetraploid triticale (A/BRR, 2n = 4x = 28). By means of differential chromosome staining, the chromosomal composition of the experimental material was characterized and the intergenomic substitution and translocation of chromosomes were detected. The PCR-RFLP analysis of the 18S/5S mitochondrial (mt) repeat and the ndhH-region of chloroplast DNA showed that these organ- elle DNA regions are in the homoplasmic state and belong to rye-type cytoplasm in secalotriticum lines and wheat-type cytoplasm in tetraploid and secondary recombinant hexaploid triticale lines. C0nclusi0ns. Cytological and molecular genetic analysis revealed significant genetic diversity of the created gene pool of wheat-rye hybrids by nuclear-cytoplasmic structure. The synthesized linear material of wheat-rye hybrids may be used in cytogenetic research and practical breeding.