Open AccessThe Mechanism Of Action Of Polyphenol On Changes In The Dynamics Of Calcium In The Synaptosomes Of The Rat Brain Against The Background Of Glutamate
Author(s) -
Khoshimov N.N.
Publication year - 2021
Publication title -
the american journal of applied sciences
Language(s) - English
Resource type - Journals
ISSN - 2689-0992
DOI - 10.37547/tajas/volume03issue03-08
Subject(s) - biophysics , glutamate receptor , calcium , chemistry , synaptosome , cytosol , polyphenol , biochemistry , voltage dependent calcium channel , intracellular , calcium channel , membrane , biology , enzyme , receptor , antioxidant , organic chemistry
The manuscript shows a short data used using fluorescent probes to study the effect of polyphenol PС-7 on changes in the dynamics of intracellular Ca2+ content in rat brain synaptosomes, depending on the site of glutamate binding on calcium channels by a specific mediator with glutamate. To measure the amount of cytosolic Ca2+ synaptosomes, we calculated using the Grinkevich equation. It has been shown that polyphenol PС-7 binds to the β1-subunit of the voltage-gated calcium channel and allosterically changes its conformation so that the conductivity for Ca2+ ions increases through the channel, the blocking effect of polyphenol PС-7 can be explained by its binding to voltage-gated calcium channels and activating them.