Open AccessHPLC-MS method development and validation for simultaneous quantitation of GZK-111 and CPG compounds in rat plasma
Author(s) -
Анна Леонидовна Подолько,
П. О. Бочков,
Г. Б. Колыванов,
А. А. Литвин,
О. Г. Грибакина,
Р. В. Шевченко,
В. П. Жердев
Publication year - 2020
Publication title -
farmakokinetika i farmakodinamika
Language(s) - English
Resource type - Journals
eISSN - 2686-8830
pISSN - 2587-7836
DOI - 10.37489/2587-7836-2019-4-28-36
Subject(s) - detection limit , chromatography , extraction (chemistry) , high performance liquid chromatography , linear range , quantitative analysis (chemistry) , chemistry
The technique of quantitative determination of GZK-111 and CPG compounds in rat blood plasma has been developed. The analysis was carried out using the combined method of high-performance liquid chromatography with mass spectrometric detection. The method was linear in the concentration range of 25-1000 ng/ml for both compounds. Percentage of GZK-111 extraction from rat blood plasma was 57.8 %. The lower limit of detection for GZK- 111 compound was 25 ng/ml. Percentage of extraction of CPG compound was 42.5 %. The lower limit of detection of CPG compound was also 25 ng/ml.