Open AccessStability of randomly amplified polymorphic DNA fingerprinting in genotyping clinical isolates ofHelicobacter pylori
Author(s) -
Fengchan Han,
Han-Chong Ng,
Bow Ho
Publication year - 2003
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v9.i9.2021
Subject(s) - rapd , genotyping , biology , southern blot , primer (cosmetics) , polymerase chain reaction , microbiology and biotechnology , genetics , genotype , dna profiling , dna , agarose gel electrophoresis , blot , gene , chemistry , population , genetic diversity , demography , organic chemistry , sociology
H pylori genomes are highly diversified. This project was designed to genotype H pylori isolates by the polymerase chain reaction (PCR)-based randomly amplified polymorphic DNA (RAPD) fingerprinting technique and to verify its stability by Southern blotting and DNA sequencing.