Open AccessIsolation of a novel member of small G protein superfamily and its expression in colon cancer
Author(s) -
Wei Yan,
Wenliang Wang,
Feng Zhu,
Shengquan Chen,
Qinglong Li,
Li Wang
Publication year - 2003
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v9.i8.1719
Subject(s) - microbiology and biotechnology , complementary dna , biology , polyclonal antibodies , fusion protein , gene , rapid amplification of cdna ends , exon , coding region , expression vector , recombinant dna , antibody , molecular cloning , genetics
APMCF1 is a novel human gene whose transcripts are up-regulated in apoptotic MCF-7 cells. In order to learn more about this gene's function in other tumors, we cloned its full length cDNA and prepared its polyclonal antibody to investigate its expression in colon cancers with immunohistochemistry.